SALM was developed by Ken Risko and Barry H. Margolin in November, 1991. Before you execute salm.exe file, you need to first create an input data file, salm.in. For example, saml.in may have following format. ---------------------------------- Test data set # 2 6 0.0,3, 15, , 21, , 29, 10.0,3, 16, , 18, , 21, 33.3,3, 16, , 26, , 33, 100.0,3, 67, , 51,C, 70, 333.0,3, 33,T, 38, , 41, 1000.0,3, 30, , 27, , 42, ---------------------------------- Then execute salm.exe file at the dos prompt, like c:\sal>salm then the program will generate a output file salm.out, which is shown below. ----------------------------------------------------- Test data set # 2 number of doses = 6 1, 0.00, 3, 15( ), 21( ), 29( ) 2, 10.00, 3, 16( ), 18( ), 21( ) 3, 33.30, 3, 16( ), 26( ), 33( ) 4, 100.00, 3, 67( ), 51(C), 70( ) 5, 333.00, 3, 33(T), 38( ), 41( ) 6,1000.00, 3, 30( ), 27( ), 42( ) Rejecting plate count 51 at dose 100.00 because background code = C Rejecting plate count 33 at dose 333.00 because background code = T Rejecting dose 6 because depressed mean response of 33.00 is substantially below peak response of 68.50 *** ANALYSED DATA *** No. Dose Plates --- ---- -------------- 1 0.00 15 ( ) 21 ( ) 29 ( ) 2 10.00 16 ( ) 18 ( ) 21 ( ) 3 33.30 16 ( ) 26 ( ) 33 ( ) 4 100.00 67 ( ) 70 ( ) 5 333.00 38 ( ) 41 ( ) Fitted model = (16.750088 + 0.553385 x dose) x (2 - exp( 0.001768 x dose)) *** ANALYSED DATA *** No. Dose Plates Mean Model --- ---- -------------- -------- --------- 1 0.00 15 21 29 21.7 16.8 2 10.00 16 18 21 18.3 21.9 3 33.30 16 26 33 25.0 33.0 4 100.00 67 70 68.5 58.1 5 333.00 38 41 39.5 39.8 c = 0.00 Test for GOODNESS OF FIT p-value: 0.000 Test for MUTAGENICITY p-value: 0.000 ------------------------------------------------------------------- end of readme.